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This game retains the same gameplay-style as before, requiring the player to swim to the beat with other Microbes. However, this version introduces a new cue, a two-step swim. The path the Microbes take is also different, leading in an inward spiral culminating in a U-turn, and then moving outward. The bubbles in the latter half appear as jagged circles.
This game's new cue makes the resemblance to Munchy Monk more apparent; both games have a single, steady beat, a faster three-hit cue, and now an even faster two-hit cue.