Other than the path the microbes take, there are no major visual differences.
The game is the same gameplay-wise as the original, requiring the player to launch to the beat while listening for cues. However, the game does something unique by having a tutorial before the game starts. The tutorial teaches a new cue, two notes in quick succession. When this cue happens, the microbes will quickly launch forward twice (this is faster the the three-hit cue), requiring the player to do so as well. The song incorperates this cue while also having the other two cues as well.